The Mouse CypA (Cyclophilin A) CLIA Kit is specially designed for the precise measurement of Cyclophilin A levels in mouse serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Cyclophilin A is a key protein involved in various cellular processes, including protein folding, immune response modulation, and signal transduction.
Dysregulation of Cyclophilin A has been implicated in various diseases, such as autoimmune disorders, viral infections, and neurodegenerative conditions, highlighting its significance as a potential biomarker for disease monitoring and therapeutic interventions.Overall, the Mouse CypA CLIA Kit provides researchers with a valuable tool for studying the role of Cyclophilin A in disease pathogenesis and developing novel treatment strategies.
Product Name:
Mouse CYPA (cyclophilin A) CLIA Kit
SKU:
MOES00214
Target:
Mouse CYPA (cyclophilin A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CYPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CYPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CYPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CYPA. You can calculate the concentration of Mouse CYPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
103-119
99-113
Average (%)
105
110
106
1:4
Range (%)
93-105
91-107
93-105
Average (%)
100
97
99
1:8
Range (%)
95-110
104-121
96-111
Average (%)
100
110
101
1:16
Range (%)
99-117
90-102
97-111
Average (%)
107
95
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
95
EDTA plasma (n=5)
93-111
101
Cell culture media (n=5)
97-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.89
192.52
716.03
97.99
192.53
729.26
Standard deviation
8.43
18.46
81.7
9.51
17.96
82.48
C V (%)
8.44
9.59
11.41
9.71
9.33
11.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CYPA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CYPA in samples. No significant cross-reactivity or interference between Mouse CYPA and analogues was observed.
