The Mouse CypB (Cyclophilin B) CLIA Kit is specifically designed for the quantitative detection of Cyclophilin B levels in mouse samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an excellent tool for a variety of research applications.Cyclophilin B is a key protein involved in protein folding and trafficking, playing a vital role in processes such as cell signaling and immune response.
Dysregulation of Cyclophilin B has been implicated in various diseases, including autoimmune disorders and neurological conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.With the Mouse CypB CLIA Kit, researchers can accurately measure Cyclophilin B levels in mouse samples, enabling further investigation into the role of this protein in disease pathology and treatment development.
Product Name:
Mouse CYPB (Cyclophilin B) CLIA Kit
SKU:
MOES00215
Target:
Mouse CYPB (Cyclophilin B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CYPB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CYPB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CYPB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CYPB. You can calculate the concentration of Mouse CYPB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-115
94-109
99-111
Average (%)
105
101
105
1:4
Range (%)
86-98
93-105
88-99
Average (%)
92
99
93
1:8
Range (%)
96-111
86-97
98-109
Average (%)
103
91
104
1:16
Range (%)
92-106
85-96
99-114
Average (%)
99
90
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
98
EDTA plasma (n=5)
89-101
95
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.0
288.78
666.79
101.06
267.6
620.98
Standard deviation
11.44
33.44
75.88
9.56
25.96
51.54
C V (%)
11.56
11.58
11.38
9.46
9.7
8.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CYPB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CYPB in samples. No significant cross-reactivity or interference between Mouse CYPB and analogues was observed.
