The Mouse D1R (Dopamine D1 Receptor) CLIA Kit is designed for the accurate detection of D1R levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.The Dopamine D1 receptor is a key protein involved in the regulation of various physiological processes, including the modulation of motor function, reward, and cognition.
Dysfunction of the D1 receptor has been linked to various neurological and psychiatric disorders, making it a critical biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse D1R CLIA Kit is a valuable tool for researchers seeking to investigate the role of the dopamine D1 receptor in various biological processes and disease states.
Product Name:
Mouse D1R (Dopamine D1 receptor) CLIA Kit
SKU:
MOES00229
Target:
Mouse D1R (Dopamine D1 receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse D1R. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse D1R and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse D1R, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse D1R. You can calculate the concentration of Mouse D1R in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
104-115
101-116
Average (%)
103
109
110
1:4
Range (%)
103-121
100-114
85-98
Average (%)
110
106
91
1:8
Range (%)
88-99
86-99
95-109
Average (%)
94
92
101
1:16
Range (%)
99-116
88-103
100-113
Average (%)
107
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
97-108
102
Cell culture media (n=5)
100-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
274.27
467.74
1954.85
285.73
510.37
1801.46
Standard deviation
26.38
39.06
194.51
31.69
49.15
115.47
C V (%)
9.62
8.35
9.95
11.09
9.63
6.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse D1R concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse D1R in samples. No significant cross-reactivity or interference between Mouse D1R and analogues was observed.
