The Mouse DCN (Decorin) CLIA Kit is specifically designed for the precise measurement of decorin levels in mouse serum, plasma, and cell culture supernatants. This high-quality kit offers unparalleled sensitivity and specificity, guaranteeing consistent and accurate results for various research applications.Decorin, a member of the small leucine-rich proteoglycan family, plays a critical role in extracellular matrix organization and regulation of cell signaling pathways. Its dysregulation has been linked to various diseases, including cancer, fibrosis, and inflammation.
Therefore, the Mouse DCN CLIA Kit serves as a valuable tool for studying the role of decorin in these conditions and developing novel therapeutic interventions.Overall, the Mouse DCN (Decorin) CLIA Kit from Assay Genie provides researchers with a reliable and efficient solution for analyzing decorin levels in mouse samples, advancing our understanding of the protein's function in health and disease.
Product Name:
Mouse DCN (Decorin) CLIA Kit
SKU:
MOES00220
Target:
Mouse DCN (Decorin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse DCN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DCN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DCN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse DCN. You can calculate the concentration of Mouse DCN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
93-109
98-113
Average (%)
94
100
104
1:4
Range (%)
85-95
94-108
88-100
Average (%)
90
102
94
1:8
Range (%)
94-107
99-113
87-99
Average (%)
100
107
94
1:16
Range (%)
92-106
103-119
87-101
Average (%)
100
109
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
102
EDTA plasma (n=5)
85-99
92
Cell culture media (n=5)
85-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.18
24.43
94.61
10.56
25.07
92.37
Standard deviation
1.1
2.3
7.69
1.34
1.83
10.05
C V (%)
10.81
9.41
8.13
12.69
7.3
10.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse DCN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse DCN in samples. No significant cross-reactivity or interference between Mouse DCN and analogues was observed.
