The Mouse Desmin CLIA Kit is a highly sensitive and specific assay designed to measure levels of desmin in mouse samples. Desmin is a key component of the intermediate filament network in muscle cells, playing a critical role in maintaining cell structure and function.This kit is ideal for researchers studying muscle development, regeneration, and diseases such as muscular dystrophy. With its reliable and reproducible results, the Mouse Desmin CLIA Kit is indispensable for investigating the role of desmin in various physiological and pathological processes in mouse models.
Overall, the Mouse Desmin CLIA Kit from Assay Genie is a valuable tool for understanding the biology of desmin and its implications in muscle-related disorders, providing researchers with accurate and insightful data for their studies.
Product Name:
Mouse Des (Desmin) CLIA Kit
SKU:
MOES00223
Target:
Mouse Des (Desmin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Des. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Des and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Des, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Des. You can calculate the concentration of Mouse Des in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
84-97
102-120
Average (%)
95
91
109
1:4
Range (%)
98-114
96-110
88-101
Average (%)
105
104
94
1:8
Range (%)
92-106
87-99
103-117
Average (%)
97
93
108
1:16
Range (%)
97-111
91-105
96-110
Average (%)
104
98
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.82
276.24
858.42
111.12
291.34
799.15
Standard deviation
8.77
27.68
84.04
12.21
22.81
50.99
C V (%)
8.13
10.02
9.79
10.99
7.83
6.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Des concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Des in samples. No significant cross-reactivity or interference between Mouse Des and analogues was observed.
