The Mouse DLAT (Dihydrolipoyl transacetylase) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of DLAT levels in mouse samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.DLAT is an essential enzyme involved in the citric acid cycle and plays a key role in energy metabolism. Dysregulation of DLAT has been implicated in various diseases including metabolic disorders and neurodegenerative conditions.
Therefore, measuring DLAT levels can provide valuable insights into the pathophysiology of these diseases and aid in the development of potential therapeutic strategies.Overall, the Mouse DLAT CLIA Kit is a valuable research tool for investigating the role of DLAT in health and disease, offering researchers a reliable and accurate method for quantifying DLAT levels in mouse samples.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse DLAT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DLAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DLAT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse DLAT. You can calculate the concentration of Mouse DLAT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-120
97-114
99-116
Average (%)
110
105
106
1:4
Range (%)
86-100
87-100
100-118
Average (%)
91
95
109
1:8
Range (%)
89-100
92-106
99-112
Average (%)
95
98
105
1:16
Range (%)
86-98
101-120
99-111
Average (%)
92
109
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
104
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.83
239.29
744.07
99.9
222.49
685.54
Standard deviation
10.85
28.67
78.8
8.76
23.67
49.63
C V (%)
11.82
11.98
10.59
8.77
10.64
7.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse DLAT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse DLAT in samples. No significant cross-reactivity or interference between Mouse DLAT and analogues was observed.
