The Mouse DMD (Dystrophin) CLIA Kit is specifically designed for the quantitative measurement of dystrophin levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and accuracy, ensuring reliable and reproducible results for a variety of research applications.Dystrophin is a critical protein involved in maintaining the structural integrity of muscle cells. Mutations in the dystrophin gene are associated with Duchenne muscular dystrophy, a severe and progressive muscle-wasting disorder.
The ability to accurately measure dystrophin levels is essential for understanding disease progression, evaluating potential treatments, and monitoring therapeutic efficacy.With the Mouse DMD CLIA Kit, researchers can confidently study the role of dystrophin in muscle health and disease, opening up new possibilities for developing targeted therapies and advancing our understanding of muscular dystrophy and related conditions.
Product Name:
Mouse DMD (Dystrophin) CLIA Kit
SKU:
MOES00232
Target:
Mouse DMD (Dystrophin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse DMD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DMD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DMD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse DMD. You can calculate the concentration of Mouse DMD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
86-96
102-118
Average (%)
92
91
109
1:4
Range (%)
88-103
86-98
90-100
Average (%)
94
93
95
1:8
Range (%)
101-116
92-104
95-109
Average (%)
109
97
101
1:16
Range (%)
94-108
95-110
100-114
Average (%)
101
102
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
84-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
197.83
523.78
1655.9
197.13
516.92
1794.09
Standard deviation
16.3
59.76
163.6
23.32
36.65
136.35
C V (%)
8.24
11.41
9.88
11.83
7.09
7.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse DMD concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse DMD in samples. No significant cross-reactivity or interference between Mouse DMD and analogues was observed.
