The Mouse DPD (Deoxypyridinoline) ELISA Kit is specifically designed for the accurate measurement of DPD levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with dependable and consistent results for a variety of research applications.DPD is an important biomarker for bone health and turnover, as it is a cross-linking amino acid that is heavily present in bones and cartilage. Monitoring DPD levels can provide valuable insights into bone metabolism, mineral density, and bone resorption, making this kit an essential tool for studies related to osteoporosis, bone diseases, and bone remodeling processes.
With its ease of use, fast assay time, and reliable performance, the Mouse DPD ELISA Kit from AssayGenie is an invaluable resource for researchers looking to further understand bone health and related conditions in mouse models. Order yours today and take the next step in your bone research studies.
Product Name:
Mouse DPD (Deoxypyridinoline) ELISA Kit
SKU:
MOES00955
Target:
Mouse DPD (Deoxypyridinoline)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DPD. During the reaction, DPD in the sample or standard competes with a fixed amount of DPD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DPD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DPD in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
86-101
100-115
Average (%)
99
93
106
1:4
Range (%)
87-97
89-103
95-110
Average (%)
92
95
102
1:8
Range (%)
88-102
93-107
98-109
Average (%)
93
99
103
1:16
Range (%)
87-102
93-108
94-106
Average (%)
94
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
100
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.9
28.6
91.8
11.1
30.3
87.2
Standard deviation
0.8
1.5
3.7
0.7
1.2
4.4
C V (%)
7.34
5.24
4.03
6.31
3.96
5.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of DPD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes DPD in samples. No significant cross-reactivity or interference between DPD and analogues was observed.
