Mouse ECM1 (Extracellular Matrix Protein 1) CLIA Kit
The Mouse ECM1 (Extracellular Matrix Protein 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of ECM1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an invaluable tool for research in fields such as cancer, cardiovascular diseases, and skin disorders.ECM1 is a key component of the extracellular matrix, playing a crucial role in cell adhesion, migration, and tissue development.
Dysregulation of ECM1 expression has been linked to various diseases, making it a valuable biomarker for studying disease progression and potential therapeutic interventions.Overall, the Mouse ECM1 CLIA Kit offers a comprehensive solution for researchers looking to investigate the role of ECM1 in health and disease, providing accurate and quantitative data for further analysis and experimentation.
Product Name:
Mouse ECM1 (Extracellular Matrix Protein 1) CLIA Kit
SKU:
MOES00251
Target:
Mouse ECM1 (Extracellular Matrix Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ECM1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ECM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ECM1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ECM1. You can calculate the concentration of Mouse ECM1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
93-108
87-98
Average (%)
103
99
93
1:4
Range (%)
91-103
86-102
91-102
Average (%)
97
93
97
1:8
Range (%)
96-114
96-109
92-106
Average (%)
104
102
99
1:16
Range (%)
85-99
93-107
92-110
Average (%)
92
101
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
105
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
97-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.76
48.78
204.32
26.21
52.31
222.36
Standard deviation
3.24
5.8
18.98
2.2
4.17
24.33
C V (%)
12.11
11.89
9.29
8.39
7.97
10.94
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ECM1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ECM1 in samples. No significant cross-reactivity or interference between Mouse ECM1 and analogues was observed.
