Mouse EGR2 (Early Growth Response Protein 2) CLIA Kit
The Mouse EGR2 (Early Growth Response Protein 2) CLIA Kit is specifically designed for the precise measurement of EGR2 levels in mouse samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.EGR2 is a key transcription factor that regulates gene expression and is involved in processes such as cell growth, differentiation, and development. Abnormalities in EGR2 have been linked to various diseases including neuropathies, cancers, and inflammatory disorders, highlighting its importance as a biomarker for investigating these conditions and exploring potential therapeutic interventions.
Overall, the Mouse EGR2 CLIA Kit provides researchers with a reliable tool for studying the role of EGR2 in biological processes and disease pathogenesis, ultimately advancing our understanding of complex molecular mechanisms and potential treatment targets.
Product Name:
Mouse EGR2 (Early Growth Response Protein 2) CLIA Kit
SKU:
MOES00234
Target:
Mouse EGR2 (Early Growth Response Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse EGR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EGR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EGR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse EGR2. You can calculate the concentration of Mouse EGR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
87-102
102-115
Average (%)
96
93
109
1:4
Range (%)
88-104
96-112
89-103
Average (%)
95
103
95
1:8
Range (%)
91-103
101-118
99-116
Average (%)
97
108
106
1:16
Range (%)
95-111
89-104
89-103
Average (%)
101
97
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
103
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
101-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
218.56
539.36
1978.6
216.38
539.71
2128.72
Standard deviation
25.62
64.4
135.34
23.87
61.1
214.36
C V (%)
11.72
11.94
6.84
11.03
11.32
10.07
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse EGR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse EGR2 in samples. No significant cross-reactivity or interference between Mouse EGR2 and analogues was observed.
