Mouse EMILIN1 (Elastin Microfibril Interface Located Protein 1) CLIA Kit
The Mouse Emilin1 (Elastin Microfibril Interface Located Protein 1) CLIA Kit (MOES00238) is a specialized assay designed for the accurate detection and quantification of Emilin1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.Emilin1 is a key protein located at the interface between elastin and microfibrils in the extracellular matrix, playing a crucial role in tissue development and maintenance. Studies have shown that Emilin1 is involved in processes such as cell adhesion, migration, and angiogenesis, making it a valuable biomarker for studying various physiological and pathological conditions.
By using the Mouse Emilin1 CLIA Kit, researchers can gain valuable insights into the role of Emilin1 in different biological processes and diseases, allowing for the development of novel therapeutic strategies and potential biomarkers for diagnostic purposes. This kit is essential for researchers looking to investigate the function and regulation of Emilin1 in mouse models, advancing our understanding of extracellular matrix biology and its implications in health and disease.
Product Name:
Mouse EMILIN1 (Elastin Microfibril Interface Located Protein 1) CLIA Kit
SKU:
MOES00238
Target:
Mouse EMILIN1 (Elastin Microfibril Interface Located Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse EMILIN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EMILIN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EMILIN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse EMILIN1. You can calculate the concentration of Mouse EMILIN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
101-116
92-108
Average (%)
109
108
99
1:4
Range (%)
94-104
100-116
101-118
Average (%)
99
108
108
1:8
Range (%)
97-109
99-115
90-105
Average (%)
103
107
98
1:16
Range (%)
87-99
101-116
92-106
Average (%)
93
109
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
103
EDTA plasma (n=5)
100-110
105
Cell culture media (n=5)
97-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.72
54.22
235.36
27.59
51.96
216.55
Standard deviation
2.55
5.81
17.79
3.38
5.78
16.98
C V (%)
9.91
10.72
7.56
12.25
11.12
7.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse EMILIN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse EMILIN1 in samples. No significant cross-reactivity or interference between Mouse EMILIN1 and analogues was observed.
