The Mouse Endothelin-3 (EDN3) ELISA Kit is specifically designed for the accurate detection of endothelin-3 levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an ideal tool for a variety of research applications.Endothelin-3 is a key molecule involved in the regulation of blood vessel constriction and cell proliferation. Its dysregulation has been implicated in various diseases, including cardiovascular disorders, pulmonary hypertension, and cancer.
By measuring endothelin-3 levels, researchers can gain valuable insights into the pathophysiology of these conditions and potentially identify new therapeutic targets.This Mouse Endothelin-3 (EDN3) ELISA Kit from AssayGenie provides researchers with a powerful tool for studying the role of endothelin-3 in disease progression and evaluating potential treatment options. With its user-friendly protocol and reliable performance, this kit is a valuable addition to any laboratory conducting research in the field of vascular biology and cardiovascular health.
Product Name:
Mouse Endothelin 3 (EDN3) ELISA Kit
Product Code:
MODL00403
Size:
96 Assays
Target:
EDN3
Synonyms:
ET3, PPET3, Preproendothelin-3
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
