The Mouse EpCAM (Epithelial Cell Adhesion Molecule) CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of EpCAM levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit offers precise and reproducible results, making it perfect for a variety of research applications.EpCAM is a critical protein involved in cell adhesion and signaling, playing a key role in epithelial cell biology and tumor progression.
Its levels have been linked to various diseases, including cancer, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.With its high sensitivity and specificity, the Mouse EpCAM CLIA Kit from AssayGenie provides researchers with a powerful tool for investigating EpCAM biology and its implications in disease pathology. Get accurate and reliable results for your research with this advanced kit.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse EPCAM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EPCAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EPCAM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse EPCAM. You can calculate the concentration of Mouse EPCAM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
100-114
101-117
Average (%)
93
106
109
1:4
Range (%)
101-115
91-106
90-106
Average (%)
108
98
97
1:8
Range (%)
89-104
89-106
101-112
Average (%)
95
97
107
1:16
Range (%)
86-98
87-98
86-101
Average (%)
92
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
100
EDTA plasma (n=5)
93-107
101
Cell culture media (n=5)
100-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.13
149.13
357.46
50.23
156.46
376.98
Standard deviation
5.47
16.58
36.0
5.27
11.41
36.0
C V (%)
9.92
11.12
10.07
10.49
7.29
9.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse EPCAM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse EPCAM in samples. No significant cross-reactivity or interference between Mouse EPCAM and analogues was observed.
