Mouse EPCR (Endothelial Protein C Receptor) CLIA Kit
The Mouse EPCR (Endothelial Protein C Receptor) CLIA Kit is a highly sensitive assay designed for the precise measurement of EPCR levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and accuracy, ensuring consistent and dependable results suitable for various research purposes.EPCR is a key protein involved in regulating blood coagulation and inflammation, playing a crucial role in maintaining vascular integrity and preventing thrombosis. Dysregulation of EPCR has been linked to various diseases, including sepsis, thrombosis, and inflammatory disorders, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Mouse EPCR CLIA Kit provides researchers with a valuable tool for investigating the role of EPCR in various physiological and pathological processes, ultimately contributing to advancements in biomedical research and potential clinical applications.
Product Name:
Mouse EPCR (Endothelial Protein C Receptor) CLIA Kit
SKU:
MOES00533
Target:
Mouse EPCR (Endothelial Protein C Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse EPCR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EPCR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EPCR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse EPCR. You can calculate the concentration of Mouse EPCR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-121
92-108
91-103
Average (%)
110
99
96
1:4
Range (%)
86-101
94-109
99-116
Average (%)
93
99
107
1:8
Range (%)
88-102
95-111
95-111
Average (%)
95
102
103
1:16
Range (%)
101-118
103-117
96-109
Average (%)
109
109
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
96-109
104
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.56
307.34
884.94
118.28
332.6
834.04
Standard deviation
11.59
25.6
56.64
14.38
31.86
79.82
C V (%)
10.48
8.33
6.4
12.16
9.58
9.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse EPCR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse EPCR in samples. No significant cross-reactivity or interference between Mouse EPCR and analogues was observed.
