The Mouse ER Beta (Estrogen Receptor Beta) CLIA Kit is specifically designed for the quantitative measurement of ER Beta levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.ER Beta is a key regulator of estrogen signaling, impacting various physiological processes such as reproductive development, bone health, and cognitive function.
Dysregulation of ER Beta expression has been linked to numerous diseases, including breast cancer, osteoporosis, and Alzheimer's disease, making it a valuable biomarker for disease research and therapeutic development.Overall, the Mouse ER Beta CLIA Kit provides researchers with a reliable tool to investigate the role of ER Beta in health and disease, offering valuable insights into potential treatment strategies and disease mechanisms.
Product Name:
Mouse ER beta (Estrogen Receptor Beta) CLIA Kit
SKU:
MOES00256
Target:
Mouse ER beta (Estrogen Receptor Beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ERβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ERβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ERβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ERβ. You can calculate the concentration of Mouse ERβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
85-101
95-113
Average (%)
101
92
103
1:4
Range (%)
100-119
89-102
100-115
Average (%)
108
94
109
1:8
Range (%)
98-113
89-106
85-96
Average (%)
104
97
91
1:16
Range (%)
85-99
98-111
88-100
Average (%)
91
106
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
98-110
104
Cell culture media (n=5)
102-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
193.44
348.18
1679.62
200.28
324.69
1649.17
Standard deviation
18.67
35.83
187.11
24.41
32.6
179.92
C V (%)
9.65
10.29
11.14
12.19
10.04
10.91
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ERβ concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Mouse ERβ in samples. No significant cross-reactivity or interference between Mouse ERβ and analogues was observed.
