Mouse ERN1 (Endoplasmic Reticulum To Nucleus Signalling 1) CLIA Kit
The Mouse ERN1 (Endoplasmic Reticulum to Nucleus Signalling 1) CLIA Kit is specifically designed for the detection of ERN1 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research applications.ERN1, also known as IRE1 (Inositol-requiring enzyme 1), is a key protein involved in the endoplasmic reticulum stress response pathway. It plays a crucial role in maintaining cellular homeostasis and responding to various stress stimuli.
Dysregulation of ERN1 signaling has been implicated in a wide range of diseases, including cancer, neurodegenerative disorders, and metabolic diseases, underscoring its importance as a potential therapeutic target.By using the Mouse ERN1 CLIA Kit, researchers can gain valuable insights into the role of ERN1 in disease pathogenesis and potentially identify new strategies for treatment and intervention. Trust in the accuracy and precision of this kit to support your research endeavors and further our understanding of ERN1-mediated signaling pathways.
Product Name:
Mouse ERN1 (Endoplasmic Reticulum To Nucleus Signalling 1) CLIA Kit
SKU:
MOES00239
Target:
Mouse ERN1 (Endoplasmic Reticulum To Nucleus Signalling 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ERN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ERN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ERN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ERN1. You can calculate the concentration of Mouse ERN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
90-101
95-109
Average (%)
94
96
103
1:4
Range (%)
95-107
87-100
93-107
Average (%)
102
92
100
1:8
Range (%)
101-115
91-105
103-120
Average (%)
108
98
109
1:16
Range (%)
89-105
88-100
93-109
Average (%)
96
94
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-115
105
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.71
96.9
360.85
36.78
88.86
357.73
Standard deviation
4.65
8.43
28.83
4.74
8.86
34.81
C V (%)
12.33
8.7
7.99
12.89
9.97
9.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ERN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ERN1 in samples. No significant cross-reactivity or interference between Mouse ERN1 and analogues was observed.
