The Mouse ES Endostatin CLIA Kit is a cutting-edge assay designed for the precise measurement of endostatin levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Endostatin is a vital protein involved in inhibiting angiogenesis, the process of blood vessel formation, and is known for its potential role in cancer treatment and other diseases.
By accurately measuring endostatin levels, researchers can gain valuable insights into the mechanisms of angiogenesis and potentially develop new therapies for various conditions.Overall, the Mouse ES Endostatin CLIA Kit is a valuable tool for studying angiogenesis and related processes in mouse models, offering researchers a reliable and efficient way to analyze endostatin levels in biological samples.
Product Name:
Mouse ES (Endostatin) CLIA Kit
SKU:
MOES00240
Target:
Mouse ES (Endostatin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ES. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ES, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ES. You can calculate the concentration of Mouse ES in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
87-100
90-104
Average (%)
99
93
96
1:4
Range (%)
97-113
87-100
98-112
Average (%)
103
92
104
1:8
Range (%)
90-103
87-100
102-114
Average (%)
97
93
107
1:16
Range (%)
83-97
91-105
88-101
Average (%)
90
98
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
94
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
99-114
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.15
256.89
790.29
100.28
264.9
747.72
Standard deviation
12.12
18.78
53.34
12.83
21.77
75.89
C V (%)
11.64
7.31
6.75
12.79
8.22
10.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ES concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ES in samples. No significant cross-reactivity or interference between Mouse ES and analogues was observed.
