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PE/Cyanine7 Anti-Mouse F4/80 Antibody [CI:A3-1] (AGEL0562)

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SKU AGEL0562
Product Type Antibody
Antibody Type Monoclonal Antibody
Applications FC
Reactivity Mouse
Host Species Rat
Isotype IgG2a,k
Clone CI:A3-1
Conjugation PE/Cy7
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Description

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PE/Cyanine7 Anti-Mouse F4/80 Antibody [CI:A3-1]

The Mouse F4/80 Monoclonal Antibody (PE/Cyanine7 Conjugated) (CI-A3-1) is a powerful tool for researchers exploring macrophage biology and immunology. This antibody, designated as AGEL0562, is specifically designed for detecting and analyzing the F4/80 antigen, a widely used marker for mouse macrophages. The conjugation with PE/Cyanine7 allows for fluorescence-based detection, enhancing the visualization and quantification of F4/80-expressing cells in various experimental setups.Macrophages are key immune cells involved in phagocytosis, inflammation, and tissue homeostasis. By targeting the F4/80 antigen, researchers can effectively identify and study macrophage populations within tissues and in flow cytometry analyses.

This antibody is rigorously validated for its specificity and sensitivity, ensuring reliable results in immunofluorescence and flow cytometry applications.The F4/80 Monoclonal Antibody (PE/Cyanine7 Conjugated) (CI-A3-1) is an essential tool for investigating the role of macrophages in immune responses, inflammation, and disease pathology. Its high specificity and compatibility with various experimental techniques make it a valuable asset for researchers in the fields of immunology, oncology, and inflammatory diseases. Take advantage of this antibody to advance your understanding of macrophage biology and its implications in health and disease.

Product Title:PE/Cyanine7 Anti-Mouse F4/80 Antibody [CI:A3-1]
SKU:AGEL0562
Size:50 Tests, 100 Tests, 200 Tests
Clone No:CI:A3-1
Synonyms:Adhesion G protein-coupled receptor E1;Adgre1;Cell surface glycoprotein F4/80;EGF-like module receptor 1;Adgre1;Emr1; Gpf480;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG2b, κ
Isotype Control:PE/Cyanine7 Rat IgG2b, κ Isotype Control[LTF-2]
Form:Liquid
Conjugation:PE/Cyanine 7
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:Spectrum Image
Swissprot:Q61549
Gene ID:13733
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

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