Mouse FDP (Fibrinogen Degradation Product) CLIA Kit
The Mouse FDP (Fibrinogen Degradation Product) CLIA Kit is a cutting-edge research tool designed for the accurate and efficient detection of FDP levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.FDP is a key marker of fibrinolysis, the breakdown of fibrin blood clots, and is involved in various physiological and pathological processes such as coagulation disorders, thrombosis, and inflammatory diseases.
Studying FDP levels can provide valuable insights into these conditions and aid in the development of novel therapeutic strategies.With its user-friendly format and robust performance, the Mouse FDP CLIA Kit is a valuable tool for researchers seeking to advance their understanding of fibrin degradation and its implications in health and disease. Trust in this advanced kit to deliver accurate and reproducible results for your experimental needs.
Product Name:
Mouse FDP (Fibrinogen Degradation Product) CLIA Kit
SKU:
MOES00260
Target:
Mouse FDP (Fibrinogen Degradation Product)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FDP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FDP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FDP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FDP. You can calculate the concentration of Mouse FDP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
95-111
101-115
Average (%)
101
101
107
1:4
Range (%)
100-116
95-111
89-103
Average (%)
109
101
94
1:8
Range (%)
99-116
92-106
96-112
Average (%)
106
100
102
1:16
Range (%)
103-115
87-99
95-108
Average (%)
109
94
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
86-97
92
Cell culture media (n=5)
99-117
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.15
296.94
861.09
113.62
308.53
841.67
Standard deviation
12.64
24.68
53.22
11.92
28.32
87.03
C V (%)
11.91
8.31
6.18
10.49
9.18
10.34
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FDP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FDP in samples. No significant cross-reactivity or interference between Mouse FDP and analogues was observed.
