The Mouse FETUA (Fetuin A) ELISA Kit is meticulously designed for the precise and quantitative measurement of Fetuin A levels in a variety of biological samples. Fetuin A, a multifunctional glycoprotein also known as alpha-2-HS-glycoprotein, plays vital roles in regulating diverse physiological processes. Fetuin A is recognized for its involvement in various cellular functions, including inhibition of calcification, modulation of insulin signaling, and regulation of inflammatory responses. Accurate measurement of Fetuin A levels is fundamental in understanding its implications in metabolic disorders, bone health, and inflammatory conditions.
Fetuin A serves as a biomarker with diagnostic and prognostic relevance in several diseases, making it an essential target for research in biomedical and clinical settings. The Mouse FETUA ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring dependable and reproducible results. Manufactured under strict quality standards, this kit delivers robust performance and user-friendly operation, making it an excellent choice for researchers investigating the functions and significance of Fetuin A in biological systems.
Product Name:
Mouse FETUA (Fetuin A) ELISA Kit
SKU:
AEES00258
Target:
Mouse FETUA (Fetuin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 ng/mL
Detection range:
78.12-5000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FETUA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FETUA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FETUA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FETUA. You can calculate the concentration of Mouse FETUA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
96-106
94-110
Average (%)
96
101
101
1:4
Range (%)
88-102
90-102
96-110
Average (%)
94
97
102
1:8
Range (%)
86-101
92-110
96-109
Average (%)
92
100
102
1:16
Range (%)
90-101
91-106
91-106
Average (%)
96
98
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
90-100
95
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
233.87
439.74
2268.36
211.88
402.49
2405.61
Standard deviation
13.7
18.29
78.71
14.64
18.64
72.41
C V (%)
5.86
4.16
3.47
6.91
4.63
3.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FETUA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FETUA in samples. No significant cross-reactivity or interference between Mouse FETUA and analogues was observed.
