The Mouse FGF21 (Fibroblast Growth Factor-21) ELISA Kit is specifically designed for the accurate measurement of FGF21 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.FGF21 is a key player in regulating metabolic processes, including glucose and lipid metabolism.
It plays a crucial role in energy homeostasis, making it a valuable biomarker for investigating metabolic disorders such as obesity, diabetes, and fatty liver disease. This ELISA kit provides researchers with a reliable tool for studying the role of FGF21 in these conditions and exploring potential therapeutic interventions.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FGF21. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FGF21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FGF21, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FGF21. You can calculate the concentration of Mouse FGF21 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
97-112
97-109
Average (%)
93
104
102
1:4
Range (%)
90-102
81-93
82-97
Average (%)
96
86
89
1:8
Range (%)
91-104
84-98
84-94
Average (%)
96
91
89
1:16
Range (%)
88-101
85-99
87-101
Average (%)
94
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
99
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.57
248.69
885.55
106.49
258.75
964.05
Standard deviation
6.07
10.57
46.49
6.4
10.45
40.1
C V (%)
5.64
4.25
5.25
6.01
4.04
4.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FGF21 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FGF21 in samples. No significant cross-reactivity or interference between Mouse FGF21 and analogues was observed.
