The Mouse FGF4 (Fibroblast Growth Factor 4) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of FGF4 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it a valuable tool for a variety of research applications.FGF4 is a key growth factor involved in embryonic development, tissue regeneration, and cell growth. It plays a crucial role in various biological processes, including wound healing, tissue repair, and tumor growth.
By measuring FGF4 levels, researchers can gain insights into the role of this protein in different physiological and pathological conditions, making it a valuable biomarker for studying developmental processes and diseases.Overall, the Mouse FGF4 CLIA Kit offers researchers a powerful tool to study the functions of FGF4 in various biological contexts, paving the way for potential therapeutic strategies targeting this important growth factor.
Product Name:
Mouse FGF4 (Fibroblast Growth Factor 4) CLIA Kit
SKU:
MOES00109
Target:
Mouse FGF4 (Fibroblast Growth Factor 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FGF4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FGF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FGF4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FGF4. You can calculate the concentration of Mouse FGF4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
87-99
95-110
Average (%)
104
92
103
1:4
Range (%)
88-102
91-104
100-113
Average (%)
93
97
106
1:8
Range (%)
100-115
97-115
101-116
Average (%)
108
105
108
1:16
Range (%)
89-101
96-109
88-99
Average (%)
94
101
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
103
EDTA plasma (n=5)
92-108
100
Cell culture media (n=5)
84-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.69
64.34
194.7
23.43
66.79
179.51
Standard deviation
2.84
5.19
16.94
2.62
7.39
18.76
C V (%)
11.99
8.07
8.7
11.18
11.06
10.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FGF4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FGF4 in samples. No significant cross-reactivity or interference between Mouse FGF4 and analogues was observed.
