The Mouse FGF6 (Fibroblast Growth Factor 6) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of FGF6 levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for a wide range of research applications.FGF6 is a key growth factor that plays a crucial role in cell growth, development, and wound healing. It is involved in various physiological processes, including tissue repair and regeneration, making it a valuable biomarker for studying these processes and developing novel therapeutic strategies.
With its high sensitivity and specificity, the Mouse FGF6 CLIA Kit provides researchers with a powerful tool for investigating the role of FGF6 in various biological processes and diseases. Trust in the accuracy and precision of this kit to advance your research efforts and uncover new insights into the function of FGF6 in the mouse model.
Product Name:
Mouse FGF6 (Fibroblast Growth Factor 6) CLIA Kit
SKU:
MOES00110
Target:
Mouse FGF6 (Fibroblast Growth Factor 6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FGF6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FGF6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FGF6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FGF6. You can calculate the concentration of Mouse FGF6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
98-115
92-103
Average (%)
101
106
97
1:4
Range (%)
104-117
90-103
101-120
Average (%)
110
96
109
1:8
Range (%)
93-107
95-113
93-105
Average (%)
99
103
99
1:16
Range (%)
92-104
92-105
89-102
Average (%)
99
99
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
108
EDTA plasma (n=5)
85-100
91
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.25
53.76
227.85
27.72
56.24
225.52
Standard deviation
2.94
5.23
13.97
2.69
5.75
24.2
C V (%)
11.64
9.73
6.13
9.7
10.22
10.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FGF6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FGF6 in samples. No significant cross-reactivity or interference between Mouse FGF6 and analogues was observed.
