The Mouse FGF9 (Fibroblast Growth Factor 9) CLIA Kit is a highly reliable and sensitive assay designed for the accurate measurement of FGF9 levels in mouse serum, plasma, and tissue lysate samples. This kit provides precise and reproducible results, making it an invaluable tool for researchers studying the role of FGF9 in various biological processes.FGF9 is a key growth factor that plays a critical role in tissue development, cell growth, and differentiation. It is involved in a wide range of physiological processes, including embryogenesis, organogenesis, and wound healing.
Dysregulation of FGF9 has been linked to various diseases, such as cancer, skeletal disorders, and cardiovascular diseases, highlighting its importance as a potential therapeutic target.With its high sensitivity and specificity, the Mouse FGF9 CLIA Kit offers researchers a reliable and efficient method for quantifying FGF9 levels, enabling deeper insights into the molecular mechanisms underlying disease pathogenesis and facilitating the development of novel treatment strategies.
Product Name:
Mouse FGF9 (Fibroblast Growth Factor 9) CLIA Kit
SKU:
MOES00264
Target:
Mouse FGF9 (Fibroblast Growth Factor 9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FGF9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FGF9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FGF9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FGF9. You can calculate the concentration of Mouse FGF9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
96-112
97-110
Average (%)
95
102
103
1:4
Range (%)
101-117
99-114
93-105
Average (%)
107
106
98
1:8
Range (%)
91-104
90-107
99-111
Average (%)
96
98
105
1:16
Range (%)
93-107
84-97
85-97
Average (%)
100
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
92-102
97
Cell culture media (n=5)
93-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.82
90.98
407.67
55.79
86.59
407.35
Standard deviation
6.52
9.22
35.3
5.58
8.69
32.3
C V (%)
12.83
10.13
8.66
10.0
10.04
7.93
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FGF9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FGF9 in samples. No significant cross-reactivity or interference between Mouse FGF9 and analogues was observed.
