The Mouse Flt1 (VEGFR1) ELISA Kit is specifically designed for the precise measurement of Flt1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Flt1, also known as VEGFR1 (Vascular Endothelial Growth Factor Receptor 1), plays a vital role in angiogenesis by promoting the formation of blood vessels and influencing cell growth.
Its involvement in various diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions makes it a critical biomarker for understanding these diseases and exploring potential treatment options.Overall, the Mouse Flt1 (VEGFR1) ELISA Kit is a valuable tool for researchers studying angiogenesis and related diseases in mouse models, providing reliable data to support their investigations.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGFR1/FLT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGFR1/FLT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGFR1/FLT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse VEGFR1/FLT1. You can calculate the concentration of Mouse VEGFR1/FLT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-100
90-104
93-108
Average (%)
95
97
99
1:4
Range (%)
89-105
83-94
88-101
Average (%)
97
88
93
1:8
Range (%)
89-102
81-94
83-94
Average (%)
94
88
88
1:16
Range (%)
93-106
80-93
90-103
Average (%)
98
86
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
89-102
97
Cell culture media (n=5)
92-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.28
4.22
0.55
1.37
4.2
Standard deviation
0.03
0.07
0.16
0.03
0.06
0.22
C V (%)
5.56
5.47
3.79
5.45
4.38
5.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse VEGFR1/FLT1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VEGFR1/FLT1 in samples. No significant cross-reactivity or interference between Mouse VEGFR1/FLT1 and analogues was observed.
