The Mouse FPR2 (Formyl Peptide Receptor 2) CLIA Kit is specifically designed for the precise detection of FPR2 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.FPR2 is a key receptor involved in the immune response, specifically in the recognition of formyl peptides released by bacteria and mitochondria.
Its role in inflammatory processes and the regulation of immune cell functions makes it a crucial target for studying conditions related to immune response dysregulation.With its advanced technology and proven performance, the Mouse FPR2 CLIA Kit is an indispensable tool for investigating immune responses, inflammation, and related diseases in mouse models. Get reliable data and valuable insights with this high-quality assay kit from Assay Genie.
Product Name:
Mouse FPR2 (Formyl Peptide Receptor 2) CLIA Kit
SKU:
MOES00270
Target:
Mouse FPR2 (Formyl Peptide Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FPR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FPR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FPR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FPR2. You can calculate the concentration of Mouse FPR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
94-112
89-102
Average (%)
93
102
96
1:4
Range (%)
91-107
94-108
94-109
Average (%)
97
102
102
1:8
Range (%)
98-115
92-105
98-111
Average (%)
105
99
104
1:16
Range (%)
87-103
86-96
84-97
Average (%)
94
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-101
92
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.32
72.77
367.79
37.73
76.79
402.9
Standard deviation
3.92
6.64
30.05
4.83
8.35
26.75
C V (%)
9.72
9.12
8.17
12.8
10.87
6.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FPR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FPR2 in samples. No significant cross-reactivity or interference between Mouse FPR2 and analogues was observed.
