The Mouse FSH (Follicle Stimulating Hormone) CLIA Kit is a powerful tool for accurately measuring levels of FSH in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.FSH is a key hormone that plays a vital role in the reproductive system, specifically in the regulation of ovarian follicle development and sperm production.
Dysregulation of FSH levels can lead to fertility issues and other reproductive disorders, making it an important biomarker for studying these conditions and developing potential treatments.With the Mouse FSH CLIA Kit, researchers can conveniently and reliably measure FSH levels in mouse samples, allowing for a deeper understanding of reproductive health and potential therapeutic interventions.
Product Name:
Mouse FSH (follicle-stimulating hormone) CLIA Kit
SKU:
MOES00268
Target:
Mouse FSH (follicle-stimulating hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FSH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FSH. You can calculate the concentration of Mouse FSH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-117
94-108
95-108
Average (%)
106
99
102
1:4
Range (%)
88-102
99-114
99-112
Average (%)
95
108
105
1:8
Range (%)
100-114
96-109
89-100
Average (%)
107
103
94
1:16
Range (%)
97-109
99-114
95-111
Average (%)
102
105
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
96
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
1.71
7.18
0.92
1.72
7.59
Standard deviation
0.12
0.13
0.46
0.1
0.19
0.48
C V (%)
12.24
7.6
6.41
10.87
11.05
6.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FSH in samples. No significant cross-reactivity or interference between Mouse FSH and analogues was observed.
