The Mouse FST (Follistatin) CLIA Kit is specifically designed for the precise measurement of follistatin levels in mouse serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Follistatin is a key regulatory protein involved in inhibiting activin, a member of the TGF-beta superfamily that plays a critical role in cell differentiation and growth. By modulating activin signaling, follistatin can impact various physiological processes, including muscle growth, tissue repair, and reproductive function.
As such, measuring follistatin levels can provide valuable insights into these biological pathways and their potential implications in different disease states.Whether investigating muscle development, reproductive health, or other related fields, the Mouse FST CLIA Kit from Assay Genie offers a reliable and user-friendly solution for detecting and quantifying follistatin levels in mouse samples. Trust in this cutting-edge assay to support your research efforts and uncover new discoveries in the realm of follistatin biology.
Product Name:
Mouse FST (Follistatin) CLIA Kit
SKU:
MOES00269
Target:
Mouse FST (Follistatin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 ng/mL
Detection range:
62.50-4000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FST. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FST, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FST. You can calculate the concentration of Mouse FST in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
94-108
89-100
Average (%)
105
100
95
1:4
Range (%)
86-100
92-106
98-114
Average (%)
91
98
104
1:8
Range (%)
94-110
95-110
86-99
Average (%)
101
103
92
1:16
Range (%)
100-115
89-104
87-100
Average (%)
105
96
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-112
107
EDTA plasma (n=5)
88-102
96
Cell culture media (n=5)
99-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
184.62
437.88
1365.37
181.92
433.23
1281.43
Standard deviation
17.87
35.51
152.38
16.55
50.51
86.88
C V (%)
9.68
8.11
11.16
9.1
11.66
6.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FST concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FST in samples. No significant cross-reactivity or interference between Mouse FST and analogues was observed.
