The Mouse FVIII (Coagulation Factor VIII) CLIA Kit is a cutting-edge assay designed for the accurate detection of Factor VIII levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it indispensable for various research applications.Coagulation Factor VIII is a key protein involved in the blood clotting cascade, playing a crucial role in hemostasis. Dysregulation of Factor VIII levels is associated with bleeding disorders such as hemophilia A, making it a vital biomarker for studying these conditions and developing novel therapeutic approaches.
By utilizing the Mouse FVIII CLIA Kit, researchers can gain valuable insights into the physiological and pathological roles of Factor VIII in mouse models, ultimately advancing our understanding of hemostasis and contributing to the development of innovative treatments for bleeding disorders.
Product Name:
Mouse FVIII (Coagulation Factor VIII) CLIA Kit
SKU:
MOES00183
Target:
Mouse FVIII (Coagulation Factor VIII)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse F8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse F8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse F8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse F8. You can calculate the concentration of Mouse F8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
95-111
91-104
Average (%)
103
102
97
1:4
Range (%)
91-103
103-120
95-109
Average (%)
96
109
100
1:8
Range (%)
92-106
95-106
87-100
Average (%)
98
100
92
1:16
Range (%)
89-101
94-108
94-107
Average (%)
94
101
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
93-104
98
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
217.64
590.35
1347.16
212.8
532.03
1268.09
Standard deviation
22.9
51.83
146.84
27.49
39.96
108.55
C V (%)
10.52
8.78
10.9
12.92
7.51
8.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse F8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse F8 in samples. No significant cross-reactivity or interference between Mouse F8 and analogues was observed.
