The Mouse G-CSF (Granulocyte Colony-Stimulating Factor) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of G-CSF levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for researchers studying the role of G-CSF in various biological processes.G-CSF is a key cytokine responsible for the production and differentiation of neutrophils, playing a crucial role in immune response and inflammation.
Dysregulation of G-CSF has been implicated in inflammatory diseases, cancer, and hematopoietic disorders, highlighting its importance as a potential biomarker for disease diagnosis and therapy development.Overall, the Mouse G-CSF CLIA Kit offers researchers a valuable tool for investigating the function of G-CSF in physiological and pathological conditions, ultimately contributing to advancements in biomedical research and clinical applications.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse G-CSF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse G-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse G-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse G-CSF. You can calculate the concentration of Mouse G-CSF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-105
99-116
96-110
Average (%)
96
106
104
1:4
Range (%)
94-107
103-115
90-107
Average (%)
100
109
98
1:8
Range (%)
97-111
103-119
95-109
Average (%)
104
109
102
1:16
Range (%)
86-98
100-113
96-109
Average (%)
91
107
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
93
EDTA plasma (n=5)
99-110
105
Cell culture media (n=5)
97-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.27
70.91
200.47
25.99
71.58
198.58
Standard deviation
2.47
5.39
15.9
2.78
5.33
12.93
C V (%)
9.06
7.6
7.93
10.7
7.45
6.51
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse G-CSF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse G-CSF in samples. No significant cross-reactivity or interference between Mouse G-CSF and analogues was observed.
