The Mouse Gal1 (Galectin-1) CLIA Kit is a reliable and accurate tool for measuring galectin-1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with dependable and reproducible results for a variety of research applications.Galectin-1 is a key protein involved in processes such as cell adhesion, inflammation, and immune response regulation. Its dysregulation has been linked to various diseases, including cancer, inflammatory disorders, and autoimmune conditions.
As a critical biomarker, galectin-1 is vital for understanding the mechanisms underlying these conditions and exploring potential therapeutic interventions.Get your Mouse Gal1 CLIA Kit from Assay Genie today and unlock new insights into the role of galectin-1 in mouse physiology and disease pathology.
Product Name:
Mouse GAL1 (Galectin 1) CLIA Kit
SKU:
MOES00274
Target:
Mouse GAL1 (Galectin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GAL1. You can calculate the concentration of Mouse GAL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
86-100
101-116
Average (%)
94
91
108
1:4
Range (%)
93-108
92-105
95-109
Average (%)
100
98
101
1:8
Range (%)
104-117
88-103
87-98
Average (%)
110
94
93
1:16
Range (%)
87-98
90-102
87-99
Average (%)
92
97
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
0.86
4.08
0.45
0.82
4.29
Standard deviation
0.05
0.1
0.26
0.05
0.08
0.35
C V (%)
10.2
11.63
6.37
11.11
9.76
8.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GAL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GAL1 in samples. No significant cross-reactivity or interference between Mouse GAL1 and analogues was observed.
