The Mouse GAL12 (Galectin-12) CLIA Kit is a highly accurate assay designed for the quantitative detection of GAL12 levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Galectin-12 is a key protein involved in regulating inflammation and immune response, making it a valuable biomarker for studying inflammatory diseases, autoimmune disorders, and metabolic syndromes.
By measuring GAL12 levels, researchers can gain insights into the pathogenesis of these conditions and potentially identify new therapeutic targets.Overall, the Mouse GAL12 CLIA Kit from Assay Genie is a powerful tool for investigating the role of Galectin-12 in various physiological and pathological processes, providing researchers with valuable data to advance their research efforts.
Product Name:
Mouse GAL12 (Galectin 12) CLIA Kit
SKU:
MOES00282
Target:
Mouse GAL12 (Galectin 12)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GAL12. You can calculate the concentration of Mouse GAL12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
100-113
90-100
Average (%)
108
106
95
1:4
Range (%)
92-107
87-98
90-102
Average (%)
99
93
96
1:8
Range (%)
97-108
84-98
92-104
Average (%)
102
90
97
1:16
Range (%)
92-106
84-98
91-102
Average (%)
98
91
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
101
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
87-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.83
72.84
274.17
39.09
78.05
274.93
Standard deviation
4.59
6.05
19.03
4.14
9.05
28.32
C V (%)
10.97
8.31
6.94
10.59
11.6
10.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GAL12 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GAL12 in samples. No significant cross-reactivity or interference between Mouse GAL12 and analogues was observed.
