The Mouse Galectin-4 CLIA Kit is specifically designed for the precise quantification of galectin-4 levels in mouse serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results for various research purposes.Galectin-4 is a key protein involved in cell adhesion and signaling processes, playing a vital role in the regulation of immune responses and inflammation. Its dysregulation has been linked to various diseases, including inflammatory bowel disease, cancer, and autoimmune disorders, making it a valuable biomarker for investigating these conditions and developing potential therapeutic interventions.
Overall, the Mouse Galectin-4 CLIA Kit is a valuable tool for researchers studying the role of galectin-4 in different physiological and pathological processes in mouse models. Its reliable performance and accuracy make it an essential component of any laboratory conducting research in the field of immunology and inflammation.
Product Name:
Mouse GAL4 (Galectin 4) CLIA Kit
SKU:
MOES00277
Target:
Mouse GAL4 (Galectin 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GAL4. You can calculate the concentration of Mouse GAL4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-113
93-106
93-111
Average (%)
107
100
101
1:4
Range (%)
94-107
99-116
92-110
Average (%)
99
107
100
1:8
Range (%)
95-107
89-104
88-100
Average (%)
100
95
93
1:16
Range (%)
86-100
86-101
100-117
Average (%)
93
93
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
104
EDTA plasma (n=5)
93-106
98
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.06
257.07
930.55
95.52
250.42
885.36
Standard deviation
9.45
18.82
83.1
8.13
22.06
93.23
C V (%)
9.17
7.32
8.93
8.51
8.81
10.53
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GAL4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GAL4 in samples. No significant cross-reactivity or interference between Mouse GAL4 and analogues was observed.
