The Mouse Gal6 (Galectin-6) CLIA Kit is a powerful tool designed for the precise measurement of Galectin-6 levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Galectin-6 is a key protein involved in various biological processes, including cell adhesion, inflammation, and immune response regulation.
Dysregulation of Galectin-6 has been linked to inflammatory diseases, autoimmune disorders, and cancer progression, highlighting its importance as a potential biomarker for disease diagnosis and prognosis.With its user-friendly protocol and reliable performance, the Mouse Gal6 CLIA Kit is a valuable asset for researchers seeking to explore the role of Galectin-6 in disease pathogenesis and therapeutic development.
Product Name:
Mouse GAL6 (Galectin 6) CLIA Kit
SKU:
MOES00278
Target:
Mouse GAL6 (Galectin 6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GAL6. You can calculate the concentration of Mouse GAL6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
86-101
96-108
Average (%)
93
94
103
1:4
Range (%)
104-116
92-107
99-111
Average (%)
110
98
105
1:8
Range (%)
93-105
89-105
94-109
Average (%)
99
96
101
1:16
Range (%)
102-117
100-117
100-117
Average (%)
110
109
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
108
EDTA plasma (n=5)
92-105
99
Cell culture media (n=5)
93-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.6
263.16
913.96
108.85
288.16
982.2
Standard deviation
12.6
23.34
76.59
12.41
32.39
63.35
C V (%)
12.65
8.87
8.38
11.4
11.24
6.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GAL6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GAL6 in samples. No significant cross-reactivity or interference between Mouse GAL6 and analogues was observed.
