The Mouse Gal9 (Galectin-9) CLIA Kit is specifically designed for the precise measurement of Galectin-9 levels in mouse serum, plasma, and cell culture supernatants. This CLIA kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Galectin-9 is a vital protein that plays a crucial role in immune regulation and inflammation. It is known to modulate T cell functions, regulate immune responses, and participate in various autoimmune diseases and inflammatory conditions.
Understanding Galectin-9 levels can provide valuable insights into the immune system and help identify potential therapeutic targets for immune-related disorders.With its high performance and reliability, the Mouse Gal9 CLIA Kit is an invaluable tool for researchers studying immune responses, inflammation, and autoimmune diseases in mouse models. Get accurate and reproducible results with this advanced CLIA kit to advance your research in immunology and beyond.
Product Name:
Mouse GAL9 (Galectin 9) CLIA Kit
SKU:
MOES00281
Target:
Mouse GAL9 (Galectin 9)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GAL9. You can calculate the concentration of Mouse GAL9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
97-111
103-120
Average (%)
107
104
109
1:4
Range (%)
85-95
93-111
94-108
Average (%)
90
101
100
1:8
Range (%)
102-114
95-109
96-109
Average (%)
109
102
103
1:16
Range (%)
89-103
95-111
96-110
Average (%)
95
102
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-114
107
EDTA plasma (n=5)
87-99
93
Cell culture media (n=5)
98-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.65
21.66
98.96
11.3
19.56
91.79
Standard deviation
1.34
2.17
9.92
1.21
1.56
7.9
C V (%)
12.58
10.02
10.02
10.71
7.98
8.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GAL9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GAL9 in samples. No significant cross-reactivity or interference between Mouse GAL9 and analogues was observed.
