The Mouse Galectin-6 (Gal6) ELISA Kit is specially designed for the precise measurement of Galectin-6 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for various research investigations.Galectin-6 is a key protein that plays a critical role in regulating immune responses and inflammatory processes. It is also involved in cell adhesion and migration, making it a crucial factor in various physiological and pathological conditions.
Studying Galectin-6 levels can provide valuable insights into autoimmune disorders, inflammatory diseases, and cancer progression, offering potential targets for therapeutic interventions.With its user-friendly protocol and reliable performance, the Mouse Galectin-6 (Gal6) ELISA Kit is an indispensable resource for researchers exploring the functions and mechanisms of Galectin-6 in mouse models. Get accurate and reproducible results to advance your research with this advanced ELISA kit.
Product Name:
Mouse Galectin 6 (GAL6) ELISA Kit
Product Code:
MODL00522
Size:
96 Assays
Target:
GAL6
Synonyms:
LGALS6, Lectin,Galactoside-Binding Soluble 6
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
