The Mouse GC (Glucagon) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of glucagon levels in mouse serum, plasma, and cell culture supernatants. This comprehensive kit provides reliable and reproducible results, making it ideal for a variety of research applications.Glucagon is a key hormone involved in regulating blood sugar levels by stimulating the liver to release glucose into the bloodstream. It plays a crucial role in metabolic processes and is essential for maintaining glucose homeostasis.
Dysregulation of glucagon levels is associated with conditions such as diabetes and metabolic disorders, making it a valuable biomarker for understanding and developing treatments for these diseases.With its easy-to-use format and exceptional performance, the Mouse GC (Glucagon) ELISA Kit from Assay Genie is a valuable tool for researchers studying glucagon biology and its implications in various health conditions.
Product Name:
Mouse GC (Glucagon) ELISA Kit
SKU:
MOES01072
Target:
Mouse GC (Glucagon)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GC. During the reaction, GC in the sample or standard competes with a fixed amount of GC on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GC. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GC in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
97-110
89-101
Average (%)
102
103
95
1:4
Range (%)
91-104
91-105
95-108
Average (%)
96
99
102
1:8
Range (%)
85-95
91-106
99-114
Average (%)
90
98
106
1:16
Range (%)
86-100
89-104
94-110
Average (%)
91
96
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
100
EDTA plasma (n=5)
92-102
97
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.4
253.5
739.4
98.9
254.2
733.7
Standard deviation
5.6
12.7
31.8
6.6
14.2
32.3
C V (%)
5.17
5.01
4.3
6.67
5.59
4.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of GC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes GC in samples. No significant cross-reactivity or interference between GC and analogues was observed.
