The Mouse GDF1 (Growth Differentiation Factor 1) CLIA Kit is a powerful tool for researchers looking to accurately measure levels of GDF1 in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it suitable for a variety of research applications.GDF1 is a key protein involved in growth and differentiation processes, playing a critical role in cell development and tissue regeneration.
Its dysregulation has been implicated in various diseases, including developmental disorders and certain cancers, highlighting its importance as a potential therapeutic target.Research using the Mouse GDF1 CLIA Kit can provide valuable insights into the role of GDF1 in various physiological and pathological processes, paving the way for the development of novel treatment strategies.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GDF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GDF1. You can calculate the concentration of Mouse GDF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
89-100
96-111
Average (%)
101
94
103
1:4
Range (%)
99-115
89-103
96-112
Average (%)
106
96
104
1:8
Range (%)
89-104
91-104
90-105
Average (%)
95
98
96
1:16
Range (%)
93-105
86-100
96-108
Average (%)
98
91
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
92
EDTA plasma (n=5)
92-104
97
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.86
92.23
300.54
35.59
94.65
298.06
Standard deviation
4.3
6.83
26.39
4.04
9.28
32.31
C V (%)
11.07
7.41
8.78
11.35
9.8
10.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GDF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GDF1 in samples. No significant cross-reactivity or interference between Mouse GDF1 and analogues was observed.
