The Mouse GDF15 (Growth Differentiation Factor 15) CLIA Kit is a powerful tool for researchers looking to accurately quantify GDF15 levels in mouse serum, plasma, and tissue culture samples. This kit offers exceptional sensitivity and specificity, ensuring robust and dependable results for a variety of experimental needs.GDF15 is a key regulator of cell growth, differentiation, and survival, with implications in various physiological and pathological processes.
Its role in metabolic diseases, cardiovascular disorders, and cancer progression makes it a valuable target for biomarker studies and therapeutic development.With the Mouse GDF15 CLIA Kit, researchers can delve deeper into GDF15 biology and its potential therapeutic applications, advancing scientific understanding and medical interventions in related fields.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF15. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GDF15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDF15, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GDF15. You can calculate the concentration of Mouse GDF15 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
101-120
90-101
Average (%)
106
110
96
1:4
Range (%)
103-118
90-105
90-105
Average (%)
110
96
98
1:8
Range (%)
99-112
102-118
96-109
Average (%)
106
109
103
1:16
Range (%)
102-119
88-101
88-101
Average (%)
110
95
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
101-114
107
Cell culture media (n=5)
85-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.76
72.06
216.66
25.36
68.17
222.8
Standard deviation
2.65
6.11
24.33
3.04
4.79
23.35
C V (%)
9.55
8.48
11.23
11.99
7.03
10.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GDF15 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GDF15 in samples. No significant cross-reactivity or interference between Mouse GDF15 and analogues was observed.
