The Mouse GDF2 (Growth Differentiation Factor 2) CLIA Kit is specifically designed for the accurate quantification of GDF2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.GDF2, also known as bone morphogenetic protein 9 (BMP-9), is a key regulator of cell growth and differentiation, playing a critical role in various cellular processes.
It is involved in bone formation, vascular development, and immune response, making it a valuable biomarker for studying these physiological pathways.This Mouse GDF2 CLIA Kit provides researchers with a reliable tool for investigating the role of GDF2 in various biological processes and disease conditions. With its ease of use and accurate results, this kit is an essential resource for advancing scientific knowledge and developing potential therapeutic interventions.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GDF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GDF2. You can calculate the concentration of Mouse GDF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
88-99
101-116
Average (%)
104
94
107
1:4
Range (%)
90-104
100-113
85-99
Average (%)
97
106
92
1:8
Range (%)
88-102
95-110
88-105
Average (%)
93
102
96
1:16
Range (%)
87-100
96-111
85-98
Average (%)
92
101
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
102
EDTA plasma (n=5)
97-111
105
Cell culture media (n=5)
94-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.65
69.98
246.72
28.79
74.39
268.13
Standard deviation
3.13
6.67
18.95
2.33
7.05
19.28
C V (%)
11.74
9.53
7.68
8.09
9.48
7.19
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GDF2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GDF2 in samples. No significant cross-reactivity or interference between Mouse GDF2 and analogues was observed.
