The Mouse GDF5 (Growth Differentiation Factor 5) CLIA Kit is a cutting-edge assay designed for the accurate detection of GDF5 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reproducible results, making it an indispensable tool for a variety of research applications.GDF5 is a critical growth factor that plays a key role in skeletal development and joint formation.
It is involved in regulating the differentiation and growth of multiple cell types, making it a vital factor in musculoskeletal conditions such as osteoarthritis and skeletal dysplasia. The Mouse GDF5 CLIA Kit provides researchers with a powerful tool for studying these conditions and exploring potential therapeutic interventions.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GDF5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDF5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GDF5. You can calculate the concentration of Mouse GDF5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-99
93-106
100-117
Average (%)
94
101
107
1:4
Range (%)
97-112
88-99
87-99
Average (%)
103
93
93
1:8
Range (%)
88-101
88-102
101-114
Average (%)
93
96
108
1:16
Range (%)
94-110
86-97
89-103
Average (%)
102
91
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
101
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
111.03
200.01
956.17
108.16
181.79
951.68
Standard deviation
10.31
21.96
82.52
11.06
14.54
70.52
C V (%)
9.29
10.98
8.63
10.23
8.0
7.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GDF5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GDF5 in samples. No significant cross-reactivity or interference between Mouse GDF5 and analogues was observed.
