SynonymsCDMP1,LAP4,SYNS2,BMP14,Radotermin,Cartilage-Derived Morphogenetic Protein-1,Bone morphogenetic protein 14,Lipopolysaccharide-associated protein 4
ReactivityMouse
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
MODL00541
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P43027
Sensitivity:
0.065ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CDMP1,LAP4,SYNS2,BMP14,Radotermin,Cartilage-Derived Morphogenetic Protein-1,Bone morphogenetic protein 14,Lipopolysaccharide-associated protein 4
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Mouse Growth Differentiation Factor 5 (GDF5) ELISA Kit is specifically designed for the accurate detection of GDF5 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results essential for various research applications. GDF5 is a key protein involved in growth and differentiation, particularly in bone and joint development.
Its role in conditions such as osteoarthritis and skeletal abnormalities makes it a valuable biomarker for studying these diseases and developing potential treatments. The Mouse GDF5 ELISA Kit is a valuable tool for researchers seeking to explore the functions and mechanisms of GDF5 in mouse models.
CDMP1, LAP4, SYNS2, BMP14, Radotermin, Cartilage-Derived Morphogenetic Protein-1, Bone morphogenetic protein 14, Lipopolysaccharide-associated protein 4
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
