The Mouse GFAP (Glial Fibrillary Acidic Protein) ELISA Kit is specially designed for the accurate quantification of GFAP levels in mouse samples including serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.GFAP is a key protein found in astrocytes, supporting the structure and function of the central nervous system. Abnormal GFAP levels are associated with various neurological disorders such as Alzheimer's disease, multiple sclerosis, and brain injuries, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
Overall, the Mouse GFAP ELISA Kit from Assay Genie provides researchers with a reliable tool for investigating the role of GFAP in neurobiology and identifying potential targets for novel treatments.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GFAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GFAP. You can calculate the concentration of Mouse GFAP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
84-98
88-98
Average (%)
101
90
93
1:4
Range (%)
88-100
80-90
89-103
Average (%)
95
86
94
1:8
Range (%)
92-109
86-97
88-104
Average (%)
99
92
95
1:16
Range (%)
92-106
82-96
89-102
Average (%)
99
88
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
89-100
95
Cell culture media (n=5)
91-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.06
154.76
377.42
55.33
148.03
410.96
Standard deviation
2.97
7.03
14.19
3.19
8.19
16.64
C V (%)
5.6
4.54
3.76
5.77
5.53
4.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse GFAP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GFAP in samples. No significant cross-reactivity or interference between Mouse GFAP and analogues was observed.
