The Mouse GHRH (Growth Hormone Releasing Hormone) CLIA Kit is specifically designed for accurate detection of GHRH levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an invaluable tool for researchers in various fields.GHRH is a vital hormone that plays a crucial role in the regulation of growth hormone release, metabolism, and energy balance in mice.
Its dysregulation is associated with various growth disorders and metabolic diseases, making it a key biomarker for studying these conditions and developing targeted therapies.This CLIA kit from Assay Genie offers a user-friendly protocol with fast and efficient results, making it a valuable asset for researchers studying growth hormone regulation and associated disorders in mouse models.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GHRH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GHRH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GHRH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GHRH. You can calculate the concentration of Mouse GHRH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
94-108
85-98
Average (%)
108
100
91
1:4
Range (%)
87-99
98-116
92-106
Average (%)
93
106
99
1:8
Range (%)
98-110
94-106
99-114
Average (%)
104
101
105
1:16
Range (%)
100-116
84-99
101-116
Average (%)
106
91
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
104
EDTA plasma (n=5)
101-113
107
Cell culture media (n=5)
101-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.57
49.73
239.32
23.5
45.21
228.23
Standard deviation
2.45
5.55
19.36
1.99
5.38
20.54
C V (%)
10.39
11.16
8.09
8.47
11.9
9.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GHRH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GHRH in samples. No significant cross-reactivity or interference between Mouse GHRH and analogues was observed.
