The Mouse GLUT1 (Glucose Transporter 1) CLIA Kit is a cutting-edge tool for accurate detection of GLUT1 levels in mouse samples. This kit offers high sensitivity and specificity, guaranteeing precise and consistent results for various research purposes.GLUT1 is a pivotal protein responsible for glucose transport in cells, playing a crucial role in metabolic processes and energy production. Dysregulation of GLUT1 has been linked to various diseases, including diabetes, cancer, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and exploring potential treatments.
With its advanced technology and reliable performance, the Mouse GLUT1 CLIA Kit is an indispensable resource for researchers seeking to unravel the complex mechanisms of glucose metabolism and its implications in health and disease. Order now to elevate your research to new heights.
Product Name:
Mouse GLUT1 (Glucose Transporter 1) CLIA Kit
SKU:
MOES00604
Target:
Mouse GLUT1 (Glucose Transporter 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GLUT1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GLUT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GLUT1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GLUT1. You can calculate the concentration of Mouse GLUT1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
98-113
85-97
Average (%)
98
106
90
1:4
Range (%)
90-106
97-113
94-106
Average (%)
97
104
100
1:8
Range (%)
94-108
92-105
100-117
Average (%)
100
99
108
1:16
Range (%)
99-111
88-102
93-107
Average (%)
105
94
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
89-104
95
Cell culture media (n=5)
98-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
202.14
568.79
1930.94
198.68
603.93
1772.53
Standard deviation
18.96
43.97
219.93
20.21
54.29
115.21
C V (%)
9.38
7.73
11.39
10.17
8.99
6.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GLUT1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GLUT1 in samples. No significant cross-reactivity or interference between Mouse GLUT1 and analogues was observed.
