Mouse Glutathione S Transferase Alpha 1 (GSTa1) ELISA Kit
The Mouse Glutathione S-Transferase Alpha 1 (GSTA1) ELISA Kit is specifically designed for the accurate and sensitive detection of GSTA1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.GSTA1 is an important enzyme involved in detoxification and defense mechanisms in the body. It plays a significant role in protecting cells from oxidative stress and is associated with various diseases such as cancer, liver diseases, and neurodegenerative disorders.
Studying GSTA1 levels can provide valuable insights into the pathogenesis of these conditions and potentially lead to the development of new therapeutic strategies.With its advanced technology and user-friendly format, the Mouse GSTA1 ELISA Kit is an essential tool for researchers interested in investigating the function and regulation of GSTA1 in mouse models. Trust in the accuracy and precision of this kit to advance your research efforts and contribute to a better understanding of GSTA1 biology.
Product Name:
Mouse Glutathione S Transferase Alpha 1 (GSTa1) ELISA Kit
Product Code:
MODL00591
Size:
96 Assays
Target:
GSTa1
Synonyms:
GSTA1, GST2, GSTA1-1, GTH1, GST class-alpha member 1, GST HA subunit 1, GST-epsilon, Glutathione S-transferase A1, N-terminally processed
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
