The Mouse GNB1 (G Protein Beta 1) CLIA Kit is specifically designed for the quantitative measurement of GNB1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring precise and accurate results for various research applications.GNB1 is a key component of G protein complexes and plays a vital role in signal transduction pathways, influencing a wide range of cellular functions. Dysregulation of GNB1 has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions, highlighting its significance as a potential therapeutic target and diagnostic marker.
With its robust performance and user-friendly protocol, the Mouse GNB1 CLIA Kit from AssayGenie is an indispensable tool for researchers investigating G protein signaling pathways and exploring the implications of GNB1 dysregulation in disease progression. Unlock new insights into cellular signaling mechanisms with this innovative assay kit.
Product Name:
Mouse GNb1 (G Protein Beta 1) CLIA Kit
SKU:
MOES00272
Target:
Mouse GNb1 (G Protein Beta 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GNb1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GNb1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GNb1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GNb1. You can calculate the concentration of Mouse GNb1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
98-113
98-114
Average (%)
106
106
106
1:4
Range (%)
104-117
99-112
93-110
Average (%)
110
105
101
1:8
Range (%)
90-102
91-108
90-106
Average (%)
96
99
97
1:16
Range (%)
92-105
87-101
86-100
Average (%)
98
95
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
94-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.5
172.77
740.85
102.69
172.45
691.27
Standard deviation
9.33
17.29
52.08
12.12
19.16
69.4
C V (%)
9.87
10.01
7.03
11.8
11.11
10.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GNb1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GNb1 in samples. No significant cross-reactivity or interference between Mouse GNb1 and analogues was observed.
