The Mouse GPC4 (Glypican-4) CLIA Kit is specifically designed for the accurate measurement of Glypican-4 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.Glypican-4 is a key protein involved in cell signaling and regulation, playing a critical role in various biological processes such as development, growth, and cell differentiation.
Dysregulation of Glypican-4 has been linked to several diseases, including cancer, diabetes, and developmental disorders.By utilizing the Mouse GPC4 CLIA Kit, researchers can gain valuable insights into the role of Glypican-4 in various physiological and pathological processes, ultimately leading to a better understanding of disease mechanisms and the development of potential therapeutic interventions.
Product Name:
Mouse GPC4 (Glypican 4) CLIA Kit
SKU:
MOES00308
Target:
Mouse GPC4 (Glypican 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GPC4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GPC4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GPC4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GPC4. You can calculate the concentration of Mouse GPC4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
89-103
89-105
Average (%)
92
97
96
1:4
Range (%)
94-107
90-105
91-104
Average (%)
102
97
97
1:8
Range (%)
87-101
99-113
88-102
Average (%)
93
105
95
1:16
Range (%)
99-116
92-103
95-107
Average (%)
107
98
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
103-116
108
Cell culture media (n=5)
96-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.93
103.78
395.72
33.51
101.95
405.88
Standard deviation
4.14
7.58
44.8
4.19
11.41
34.3
C V (%)
11.21
7.3
11.32
12.5
11.19
8.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GPC4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GPC4 in samples. No significant cross-reactivity or interference between Mouse GPC4 and analogues was observed.
