The Mouse GPX1 (Glutathione Peroxidase 1) ELISA Kit is a powerful tool for measuring GPX1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.GPX1 is an important enzyme that plays a key role in protecting cells from oxidative stress by reducing harmful hydrogen peroxide and lipid hydroperoxides. It is implicated in various disease states, including cancer, cardiovascular diseases, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With the Mouse GPX1 ELISA Kit, researchers can accurately quantify GPX1 levels in biological samples, providing valuable insights into oxidative stress levels and disease mechanisms. Trust in the reliability and accuracy of this kit for your research needs.
Product Name:
Mouse GPX1 (Glutathione Peroxidase 1) ELISA Kit
SKU:
MOES01393
Target:
Mouse GPX1 (Glutathione Peroxidase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GPX1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GPX1. You can calculate the concentration of Mouse GPX1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
95-111
88-99
Average (%)
100
101
93
1:4
Range (%)
93-106
81-96
88-100
Average (%)
99
88
94
1:8
Range (%)
90-103
83-96
87-101
Average (%)
96
88
93
1:16
Range (%)
86-100
85-98
85-99
Average (%)
93
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
89-106
96
Cell culture media (n=5)
90-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
199.87
485.76
1979.86
218.14
520.4
1797.1
Standard deviation
10.77
24.24
82.96
13.57
25.4
86.44
C V (%)
5.39
4.99
4.19
6.22
4.88
4.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse GPX1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GPX1 in samples. No significant cross-reactivity or interference between Mouse GPX1 and analogues was observed.
