Mouse GR alpha (glucocorticoid receptor alpha) CLIA Kit
The Mouse GR (Glucocorticoid Receptor) Alpha CLIA Kit is a cutting-edge assay designed for the accurate quantification of GR Alpha levels in mouse samples. This kit offers high sensitivity and specificity, allowing for precise and reliable results in a variety of research applications.The Glucocorticoid Receptor Alpha, a key transcription factor, plays a crucial role in regulating gene expression in response to glucocorticoid hormones. Dysregulation of GR Alpha has been implicated in various diseases, including inflammatory disorders, autoimmune conditions, and metabolic abnormalities.
By using the Mouse GR Alpha CLIA Kit, researchers can gain valuable insights into the role of GR Alpha in disease pathogenesis and potential therapeutic interventions. This advanced assay provides a powerful tool for studying the complex mechanisms of glucocorticoid signaling and its implications for health and disease.
Product Name:
Mouse GR alpha (glucocorticoid receptor alpha) CLIA Kit
SKU:
MOES00291
Target:
Mouse GR alpha (glucocorticoid receptor alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GRα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GRα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GRα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GRα. You can calculate the concentration of Mouse GRα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
96-109
83-96
Average (%)
99
103
90
1:4
Range (%)
84-96
93-105
100-114
Average (%)
90
99
107
1:8
Range (%)
91-107
95-108
85-98
Average (%)
99
103
91
1:16
Range (%)
85-97
92-108
97-114
Average (%)
91
99
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-104
99
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.62
51.91
208.73
26.79
57.1
193.45
Standard deviation
2.61
5.19
23.25
3.19
6.64
16.21
C V (%)
10.19
10.0
11.14
11.91
11.63
8.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GRα concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GRα in samples. No significant cross-reactivity or interference between Mouse GRα and analogues was observed.
